![]() Researchers in any field who utilize bacterial systems will find this book very useful. The book describes many experiments covering the basic microbiological experiments to the applications of microbial systems for advanced research. ![]() Important background information, representative results, step by step protocol in this book will be of great use to the students, early career researchers as well as the academicians. This book summarizes the experimental setups required for applied microbiological studies. ![]() Much advancement has been taken place in the field of research on bacterial systems. Bacteria constitute the major part of the microorganisms and possess tremendous potential in many important applications from environmental clean up to the drug discovery. Microorganisms play an important role in the maintenance of the ecosystem structure and function. Microbial Biotechnology- A Laboratory Manual for Bacterial Systems Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Plant Biotechnology : Laboratory Manual For Plant BiotechnologyĪdvanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. To help students prepare for answering viva voce questions, the book includes MCQs based on the discussed techniques. It also gives necessary knowledge of different types of stains, staining techniques, buffers, reagents and media used in the protocols. Protocols on modern molecular biology techniques-PCR, restriction enzyme digest, DNA isolation, cloning and DNA sequencing-add weightage to the book. Different types of electrophoresis and their techniques, including blotting techniques and the methodology for stripping of probes from membranes for reusing the blot, have also been dealt with. All the protocols have been explained following step-by-step method. The book lucidly deals with the extraction and characteri-zation of chromatin and techniques for studying its modifications, the gene methodology for identification of mutation and the methodology for isolation of nucleic acids from all types of organisms, such as viruses, fungi, plants and animals. ![]() ![]() Experiments relating to chromosomes study and identifying the phases of cell division are explained. This laboratory guide, intended for undergraduate and postgraduate students, includes techniques and their protocols ranging from microscopy to in vitro protein synthesis. ![]()
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